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朱作言文集（上 卷） 汪亚平 等主编 2019年版
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朱作言文集（上 卷）
作 者： 汪亚平 等 著
出版时间： 2019
内容简介
朱作言先生研究团队于20 世纪80 年代初期农业动物基因工程研究，在1985 年发表了世界第一篇鱼类基因转移研究文章，享有世界声誉。《朱作言文集（全2册）》从该团队发表的200 多篇文章中，选取了100 篇编辑成册，分上卷和下卷出版。《朱作言文集（全2册）》的主线是基于转基因鱼理论模型的建立、转基因鲤研制及品系选育、转植基因功能，以及转基因鱼食用和生态安全研究。内容共包括7 个部分：第一部分和第二部分建立了转基因鱼研究的完整理论模型，并以此为基础成功研制转“全鱼”生长激素基因黄河鲤家系，系统揭示了转植基因的整合、表达和遗传规律。第三部分从摄食、代谢、生长、免疫和游泳行为等方面，详细描述了转基因黄河鲤与野生黄河鲤的生物学特性。第四部分系统评估了转基因鱼在实验室及模拟自然环境条件下的生态学效应。第五部分系统研究了鱼类生长激素对性腺发育的调控作用、育性控制技术及研制不育转基因鱼的策略。第六部分包括草鱼全基因组草图绘制、草鱼病原病毒分子生物学和抗病草鱼基因工程育种的基础研究。第七部分主要以斑马鱼为模型，对鱼类基因工程的生物学原理、技术拓展和应用进行了全方位的探讨。为了便于广大读者阅读，英文文章后面均附有中文摘要。
目 录
上 卷
第一部分 鱼类基因转移模型建立
Novel Gene Transfer into the Fertilized Eggs of Goldfish (Carassius auratus L 1758) 3
人生长激素基因在泥鳅受精卵显微注射转移后的生物学效应 8
Introduction of Novel Genes into Fish 11
转基因鱼模型的建立 23
第二部分 外源基因的整合与表达
The β-Actin Gene of Carp (Ctenopharyngodon idella) 35
Isolation and Characterization of β-Actin Gene of Carp (Cyprinus carpio) 37
Primary-Structural and Evolutionary Analyses of the Growth-Hormone Gene from Grass
Carp (Ctenopharyngodon idellus) 54
Time Course of Foreign Gene Integration and Expression in Transgenic Fish Embryos 64
Transgenes in F4 pMThGH-Transgenic Common Carp (Cyprinus carpio L ) are Highly
Polymorphic 73
Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp 84
Characterization of Transgene Integration Pattern in F4 hGH-Transgenic Common Carp
(Cyprinus carpio L ) 87
Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for the
Selection of Site-Directed Knockout/Knockin 101
Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase
Using A Combination of Mutant Lox Sites 114
Inhibition of No Tail (ntl) Gene Expression in Zebrafish by External Guide Sequence
(EGS) Technique 128
Non-Homologous End Joining Plays A Key Role in Transgene Concatemer Formation in
Transgenic Zebrafish Embryos 137
Integration Mechanisms of Transgenes and Population Fitness of GH Transgenic Fish 157
Gene Transfer and Mutagenesis Mediated by Sleeping Beauty Transposon in Nile Tilapia
(Oreochromis niloticus) 172
Targeted Expression in Zebrafish Primordial Germ Cells by Cre/LoxP and Gal4/UAS
Systems 189
Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in
Zebrafish Using TALENs 211
Efficient Ligase 3-Dependent Microhomology-Mediated End Joining Repair of DNA
Double-Strand Breaks in Zebrafish Embryos 225
第三部分 外源基因表达的生理功能
Hormonal Replacement Therapy in Fish: Human Growth Hormone Gene Function in
Hypophysectomized Carp 249
Food Consumption and Energy Budget in MThGH-Transgenic F2 Red Carp (Cyprinus
carpio L red var ) 263
Whole-Body Amino Acid Pattern of F4 Human Growth Hormone Gene-Transgenic Red
Common Carp (Cyprinus carpio) Fed Diets with Different Protein Levels 269
Transgene for Growth Hormone in Common Carp (Cyprinus carpio L ) Promotes Thymus
Development 276
Effects of the “All-Fish” Growth Hormone Transgene Expression on Non-Specific
Immune Functions of Common Carp， Cyprinus carpio L 287
Rapid Growth Cost in “All-Fish” Growth Hormone Gene Transgenic Carp: Reduced
Critical Swimming Speed 298
Growth and Energy Budget of F2 ‘All-Fish’ Growth Hormone Gene Transgenic Common
Carp 307
Fast-Growing Transgenic Common Carp Mounting Compensatory Growth 321
Metabolism Traits of ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus
carpio L ) 334
Enzyme-Linked Immunosorbent Assay of Changes in Serum Levels of Growth
Hormone (cGH) in Common Carps (Cyprinus carpio) 350
The Hematological Response to Exhaustive Exercise in ‘All-Fish’ Growth
Hormone Transgenic Common Carp (Cyprinus carpio L ) 362
Acute and Chronic Un-Ionized Ammonia Toxicity to ‘All-Fish’ Growth Hormone
Transgenic Common Carp (Cyprinus carpio L ) 376
Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and Bone
Development in Common Carp 386
Increased Food Intake in Growth Hormone-Transgenic Common Carp (Cyprinus carpio L )
May Be Mediated by Upregulating Agouti-Related Protein (AgRP) 401
第四部分 转基因黄河鲤的生态安全研究
Progress in the Evaluation of Transgenic Fish for Possible Ecological Risk and Its
Containment Strategies 423
Reduced Swimming Abilities in Fast-Growing Transgenic Common Carp Cyprinus carpio
Associated with Their Morphological Variations 433
Elevated Ability to Compete for Limited Food Resources by ‘All-Fish’ Growth
Hormone Transgenic Common Carp Cyprinus carpio 446
Increased Mortality of Growth-Enhanced Transgenic Common Carp (Cyprinus carpio L )
Under Short-Term Predation Risk 462
Behavioral Alterations in GH Transgenic Common Carp May Explain Enhanced
Competitive Feeding Ability 471
Risk-Taking Behaviour May Explain High Predation Mortality of GH-Transgenic
Common Carp Cyprinus carpio 489
Transgenic Common Carp Do Not Have the Ability to Expand Populations 506
Rapid Growth Increases Intrinsic Predation Risk in Genetically Modified Cyprinus carpio:
Implications for Environmental Risk 518
第五部分 鱼类生殖调控与转基因鱼的育性控制研究
A Perspective on Fish Gonad Manipulation for Biotechnical Applications 535
Cloning and Expression Analysis in Mature Individuals of Two Chicken Type-II GnRH
(cGnRH-II) Genes in Common Carp (Cyprinus carpio) 546
Identification and Characterization of A Novel Splice Variant of Gonadotropin α
Subunit in the Common Carp Cyprinus carpio 560
Antisense for Gonadotropin-Releasing Hormone Reduces Gonadotropin
作 者： 汪亚平 等 著
出版时间： 2019
内容简介
朱作言先生研究团队于20 世纪80 年代初期农业动物基因工程研究，在1985 年发表了世界第一篇鱼类基因转移研究文章，享有世界声誉。《朱作言文集（全2册）》从该团队发表的200 多篇文章中，选取了100 篇编辑成册，分上卷和下卷出版。《朱作言文集（全2册）》的主线是基于转基因鱼理论模型的建立、转基因鲤研制及品系选育、转植基因功能，以及转基因鱼食用和生态安全研究。内容共包括7 个部分：第一部分和第二部分建立了转基因鱼研究的完整理论模型，并以此为基础成功研制转“全鱼”生长激素基因黄河鲤家系，系统揭示了转植基因的整合、表达和遗传规律。第三部分从摄食、代谢、生长、免疫和游泳行为等方面，详细描述了转基因黄河鲤与野生黄河鲤的生物学特性。第四部分系统评估了转基因鱼在实验室及模拟自然环境条件下的生态学效应。第五部分系统研究了鱼类生长激素对性腺发育的调控作用、育性控制技术及研制不育转基因鱼的策略。第六部分包括草鱼全基因组草图绘制、草鱼病原病毒分子生物学和抗病草鱼基因工程育种的基础研究。第七部分主要以斑马鱼为模型，对鱼类基因工程的生物学原理、技术拓展和应用进行了全方位的探讨。为了便于广大读者阅读，英文文章后面均附有中文摘要。
目 录
上 卷
第一部分 鱼类基因转移模型建立
Novel Gene Transfer into the Fertilized Eggs of Goldfish (Carassius auratus L 1758) 3
人生长激素基因在泥鳅受精卵显微注射转移后的生物学效应 8
Introduction of Novel Genes into Fish 11
转基因鱼模型的建立 23
第二部分 外源基因的整合与表达
The β-Actin Gene of Carp (Ctenopharyngodon idella) 35
Isolation and Characterization of β-Actin Gene of Carp (Cyprinus carpio) 37
Primary-Structural and Evolutionary Analyses of the Growth-Hormone Gene from Grass
Carp (Ctenopharyngodon idellus) 54
Time Course of Foreign Gene Integration and Expression in Transgenic Fish Embryos 64
Transgenes in F4 pMThGH-Transgenic Common Carp (Cyprinus carpio L ) are Highly
Polymorphic 73
Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp 84
Characterization of Transgene Integration Pattern in F4 hGH-Transgenic Common Carp
(Cyprinus carpio L ) 87
Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for the
Selection of Site-Directed Knockout/Knockin 101
Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase
Using A Combination of Mutant Lox Sites 114
Inhibition of No Tail (ntl) Gene Expression in Zebrafish by External Guide Sequence
(EGS) Technique 128
Non-Homologous End Joining Plays A Key Role in Transgene Concatemer Formation in
Transgenic Zebrafish Embryos 137
Integration Mechanisms of Transgenes and Population Fitness of GH Transgenic Fish 157
Gene Transfer and Mutagenesis Mediated by Sleeping Beauty Transposon in Nile Tilapia
(Oreochromis niloticus) 172
Targeted Expression in Zebrafish Primordial Germ Cells by Cre/LoxP and Gal4/UAS
Systems 189
Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in
Zebrafish Using TALENs 211
Efficient Ligase 3-Dependent Microhomology-Mediated End Joining Repair of DNA
Double-Strand Breaks in Zebrafish Embryos 225
第三部分 外源基因表达的生理功能
Hormonal Replacement Therapy in Fish: Human Growth Hormone Gene Function in
Hypophysectomized Carp 249
Food Consumption and Energy Budget in MThGH-Transgenic F2 Red Carp (Cyprinus
carpio L red var ) 263
Whole-Body Amino Acid Pattern of F4 Human Growth Hormone Gene-Transgenic Red
Common Carp (Cyprinus carpio) Fed Diets with Different Protein Levels 269
Transgene for Growth Hormone in Common Carp (Cyprinus carpio L ) Promotes Thymus
Development 276
Effects of the “All-Fish” Growth Hormone Transgene Expression on Non-Specific
Immune Functions of Common Carp， Cyprinus carpio L 287
Rapid Growth Cost in “All-Fish” Growth Hormone Gene Transgenic Carp: Reduced
Critical Swimming Speed 298
Growth and Energy Budget of F2 ‘All-Fish’ Growth Hormone Gene Transgenic Common
Carp 307
Fast-Growing Transgenic Common Carp Mounting Compensatory Growth 321
Metabolism Traits of ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus
carpio L ) 334
Enzyme-Linked Immunosorbent Assay of Changes in Serum Levels of Growth
Hormone (cGH) in Common Carps (Cyprinus carpio) 350
The Hematological Response to Exhaustive Exercise in ‘All-Fish’ Growth
Hormone Transgenic Common Carp (Cyprinus carpio L ) 362
Acute and Chronic Un-Ionized Ammonia Toxicity to ‘All-Fish’ Growth Hormone
Transgenic Common Carp (Cyprinus carpio L ) 376
Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and Bone
Development in Common Carp 386
Increased Food Intake in Growth Hormone-Transgenic Common Carp (Cyprinus carpio L )
May Be Mediated by Upregulating Agouti-Related Protein (AgRP) 401
第四部分 转基因黄河鲤的生态安全研究
Progress in the Evaluation of Transgenic Fish for Possible Ecological Risk and Its
Containment Strategies 423
Reduced Swimming Abilities in Fast-Growing Transgenic Common Carp Cyprinus carpio
Associated with Their Morphological Variations 433
Elevated Ability to Compete for Limited Food Resources by ‘All-Fish’ Growth
Hormone Transgenic Common Carp Cyprinus carpio 446
Increased Mortality of Growth-Enhanced Transgenic Common Carp (Cyprinus carpio L )
Under Short-Term Predation Risk 462
Behavioral Alterations in GH Transgenic Common Carp May Explain Enhanced
Competitive Feeding Ability 471
Risk-Taking Behaviour May Explain High Predation Mortality of GH-Transgenic
Common Carp Cyprinus carpio 489
Transgenic Common Carp Do Not Have the Ability to Expand Populations 506
Rapid Growth Increases Intrinsic Predation Risk in Genetically Modified Cyprinus carpio:
Implications for Environmental Risk 518
第五部分 鱼类生殖调控与转基因鱼的育性控制研究
A Perspective on Fish Gonad Manipulation for Biotechnical Applications 535
Cloning and Expression Analysis in Mature Individuals of Two Chicken Type-II GnRH
(cGnRH-II) Genes in Common Carp (Cyprinus carpio) 546
Identification and Characterization of A Novel Splice Variant of Gonadotropin α
Subunit in the Common Carp Cyprinus carpio 560
Antisense for Gonadotropin-Releasing Hormone Reduces Gonadotropin
