超清版 GB/T 44342-2024 苏铁叶枯病菌检疫鉴定方法

ICS 65.020.20
CCS B 16
中华人民共和国国家标准
GB/T 44342—2024
苏铁叶枯病菌检疫鉴定方法
Detection and identification of
Didymella glomerata （Corda） Qian Chen & L. Cai
2024-08-23 发布2025-03-01 实施
国家市场监督管理总局
国家标准化管理委员会发 布

目　　次
前言 ····································································································· Ⅲ
1 范围 ·································································································· 1
2 规范性引用文件 ······················································································ 1
3 术语和定义 ··························································································· 1
4 苏铁叶枯病菌基本信息 ··············································································· 1
5 方法原理 ······························································································ 1
6 试剂、材料和培养基 ················································································· 1
6.1 试剂和材料 ······················································································ 1
6.2 培养基 ··························································································· 2
7 仪器和用具 ··························································································· 2
7.1 仪器 ······························································································ 2
7.2 用具 ······························································································ 2
8 检疫鉴定方法 ························································································· 2
8.1 病害症状 ························································································· 2
8.2 病原菌分离培养和形态鉴定 ····································································· 2
8.3 分子生物学鉴定 ················································································· 3
9 结果判定 ······························································································ 3
10 样品和档案保存 ····················································································· 3
10.1 样品保存与处理 ················································································ 3
10.2 菌株保存与处理 ················································································ 3
10.3 结果记录与资料保存 ··········································································· 3
附录 A （资料性）　苏铁叶枯病菌的其他信息 ·························································· 4
A.1 病害情况 ························································································ 4
A.2 寄主范围 ························································································ 4
A.3 分布范围 ························································································ 4
A.4 传播方式 ························································································ 4
A.5 病害症状 ·············