目 录 上 卷 第一部分 鱼类基因转移模型建立 Novel Gene Transfer into the Fertilized Eggs of Goldfish (Carassius auratus L 1758) 3 人生长激素基因在泥鳅受精卵显微注射转移后的生物学效应 8 Introduction of Novel Genes into Fish 11 转基因鱼模型的建立 23 第二部分 外源基因的整合与表达 The β-Actin Gene of Carp (Ctenopharyngodon idella) 35 Isolation and Characterization of β-Actin Gene of Carp (Cyprinus carpio) 37 Primary-Structural and Evolutionary Analyses of the Growth-Hormone Gene from Grass Carp (Ctenopharyngodon idellus) 54 Time Course of Foreign Gene Integration and Expression in Transgenic Fish Embryos 64 Transgenes in F4 pMThGH-Transgenic Common Carp (Cyprinus carpio L ) are Highly Polymorphic 73 Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp 84 Characterization of Transgene Integration Pattern in F4 hGH-Transgenic Common Carp (Cyprinus carpio L ) 87 Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for the Selection of Site-Directed Knockout/Knockin 101 Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase Using A Combination of Mutant Lox Sites 114 Inhibition of No Tail (ntl) Gene Expression in Zebrafish by External Guide Sequence (EGS) Technique 128 Non-Homologous End Joining Plays A Key Role in Transgene Concatemer Formation in Transgenic Zebrafish Embryos 137 Integration Mechanisms of Transgenes and Population Fitness of GH Transgenic Fish 157 Gene Transfer and Mutagenesis Mediated by Sleeping Beauty Transposon in Nile Tilapia (Oreochromis niloticus) 172 Targeted Expression in Zebrafish Primordial Germ Cells by Cre/LoxP and Gal4/UAS Systems 189 Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs 211 Efficient Ligase 3-Dependent Microhomology-Mediated End Joining Repair of DNA Double-Strand Breaks in Zebrafish Embryos 225 第三部分 外源基因表达的生理功能 Hormonal Replacement Therapy in Fish: Human Growth Hormone Gene Function in Hypophysectomized Carp 249 Food Consumption and Energy Budget in MThGH-Transgenic F2 Red Carp (Cyprinus carpio L red var ) 263 Whole-Body Amino Acid Pattern of F4 Human Growth Hormone Gene-Transgenic Red Common Carp (Cyprinus carpio) Fed Diets with Different Protein Levels 269 Transgene for Growth Hormone in Common Carp (Cyprinus carpio L ) Promotes Thymus Development 276 Effects of the “All-Fish” Growth Hormone Transgene Expression on Non-Specific Immune Functions of Common Carp， Cyprinus carpio L 287 Rapid Growth Cost in “All-Fish” Growth Hormone Gene Transgenic Carp: Reduced Critical Swimming Speed 298 Growth and Energy Budget of F2 ‘All-Fish’ Growth Hormone Gene Transgenic Common Carp 307 Fast-Growing Transgenic Common Carp Mounting Compensatory Growth 321 Metabolism Traits of ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus carpio L ) 334 Enzyme-Linked Immunosorbent Assay of Changes in Serum Levels of Growth Hormone (cGH) in Common Carps (Cyprinus carpio) 350 The Hematological Response to Exhaustive Exercise in ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus carpio L ) 362 Acute and Chronic Un-Ionized Ammonia Toxicity to ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus carpio L ) 376 Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and Bone Development in Common Carp 386 Increased Food Intake in Growth Hormone-Transgenic Common Carp (Cyprinus carpio L ) May Be Mediated by Upregulating Agouti-Related Protein (AgRP) 401 第四部分 转基因黄河鲤的生态安全研究 Progress in the Evaluation of Transgenic Fish for Possible Ecological Risk and Its Containment Strategies 423 Reduced Swimming Abilities in Fast-Growing Transgenic Common Carp Cyprinus carpio Associated with Their Morphological Variations 433 Elevated Ability to Compete for Limited Food Resources by ‘All-Fish’ Growth Hormone Transgenic Common Carp Cyprinus carpio 446 Increased Mortality of Growth-Enhanced Transgenic Common Carp (Cyprinus carpio L ) Under Short-Term Predation Risk 462 Behavioral Alterations in GH Transgenic Common Carp May Explain Enhanced Competitive Feeding Ability 471 Risk-Taking Behaviour May Explain High Predation Mortality of GH-Transgenic Common Carp Cyprinus carpio 489 Transgenic Common Carp Do Not Have the Ability to Expand Populations 506 Rapid Growth Increases Intrinsic Predation Risk in Genetically Modified Cyprinus carpio: Implications for Environmental Risk 518 第五部分 鱼类生殖调控与转基因鱼的育性控制研究 A Perspective on Fish Gonad Manipulation for Biotechnical Applications 535 Cloning and Expression Analysis in Mature Individuals of Two Chicken Type-II GnRH (cGnRH-II) Genes in Common Carp (Cyprinus carpio) 546 Identification and Characterization of A Novel Splice Variant of Gonadotropin α Subunit in the Common Carp Cyprinus carpio 560 Antisense for Gonadotropin-Releasing Hormone Reduces Gonadotropin
